Figure 2.

The interruption of CNGC activity inhibits PAMP-induced phosphorylation of CBP60g. A, Treatment of LaCl₃ blocks flg22-induced phosphorylation of CBP60g. Pro35S:CBP60g-FLAG transgenic plants were pretreated with 10 µM LaCl₃ or H₂O before 2 µM flg22 treatment. B, Flg22-induced phosphorylation of CBP60g is impaired in the bik1 pbl1 mutant. C, Flg22-induced phosphorylation of CBP60g is impaired in the cngc2 mutant. Protoplasts isolated from WT or mutant plants were transfected with Pro35S:CBP60g-FLAG, and then treated with or without 2 µM flg22 for 5 min. Total protein was extracted and protein samples were separated by SDS-PAGE and subjected to anti-FLAG immunoblot. CBB staining of the filter indicates loading of the protein. The experiments were repeated 3 times with similar results.