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. 2022 Jul 15;34(10):3773–3789. doi: 10.1093/plcell/koac194

Figure 5.

Figure 5

ER family members act upstream of DA1 to regulate outer integument cell proliferation. A–E, Quantification of the number of cells and perimeter of the outer integument in seeds at 0 or 8 DAP. The plants used in the genetic crosses were: er105 (er), er105 erl2-1 (er erl2), da1-1, UBP15pro:UBP15-GFP (gUBP15), ERpro:ER-4myc (gER-4myc), and ubp15-1 (ubp15). Error bars indicate the mean ± sd (n > 14). Statistically significant differences are indicated by different lowercase letters (one-way ANOVA, P < 0.05). F, Immunoblots showing the abundance of DA1-YFP and UBP15-GFP in 2 DAP seeds from DA1pro:DA1-YFP (gDA1-YFP)- or gUBP15-GFP-expressing WT (Col-0), er105 erl1-2 (er erl1), or er105 erl2-1 (er erl2) mutant plants, respectively, using anti-GFP antibody. G, Quantitation of the relative DA1-YFP or UBP15-GFP immunoblot signals in (F) using the anti-tubulin signal as an equal loading control. Error bars indicate the mean ± sd. Statistically significant differences are indicated by *(P < 0.05) or **(P < 0.01), two-tailed Student’s t test.