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. 2022 Aug 10;34(10):4105–4116. doi: 10.1093/plcell/koac206

Figure 3.

Figure 3

CRISPR/Cas9-induced bos1 loss-of-function lines were impaired in pollen release. A, The bos1 alleles created with CRISPR/Cas9 (bos1-c1 to bos1-c3) resulted in impaired fertility. White arrows indicate siliques with reduced seed production. Delayed flower senescence was also apparent in the bos1 CRISPR/Cas9-induced alleles. Bar = 1 cm. B, Anthers of the CRISPR/Cas9-induced loss-of-function alleles of bos1 exhibited delayed dehiscence. Anthers were detached from flowers at the same developmental stage (floral stage 14 according to stages defined by Sanders et al. 1999). Bar = 50 μm. C, The proportion of open anthers in the indicated genotypes. Ten flowers of each genotype at floral stage 14 were measured. Bars represent mean ± se from three independent biological repeats. Letters above bars indicate significant differences between groups (P ≤ 0.05; one-way ANOVA, Supplemental File S1). Transcript levels for BOS1 in publicly available development and stress experiments are shown in Supplemental Figure S4 and further characterization of stress responses in the new bos1 CRISPR/Cas9-induced alleles in Supplemental Figures S5–S7.