Skip to main content
. 2022 Sep 11;12(15):6665–6681. doi: 10.7150/thno.75444

Figure 4.

Figure 4

SPMI-HIF2-1 could efficiently penetrate cell membranes and induce cell cycle arrest and apoptosis. (A) Representative images of FITC-labeled PMI-HIF1-1, SPMI2, and SPMI-HIF2-1 (10 µM) localization in HCT116 cells as determined by confocal microscopy. The FITC fluorescent group was attached to the N-terminus of peptides via aminohexanoic acid. Imaging was performed using an LSM 510 Zeiss Axiovert 200M (v4.0) confocal microscope and images were analyzed using LSM Image Viewer. Peptide stapling could effectively aid the peptides to penetrate cell membranes. (B) SPMI-HIF2-1 caused cell cycle arrest in HCT116 cancer cells. The cell cycle distribution was detected with a cell cycle staining kit. (C and D) Apoptotic response to PMI-HIF1-1, SPMI2, and SPMI-HIF2-1 in HCT116 cells as analyzed by flow cytometry. HCT116 cells were exposed to PMI-HIF1-1, SPMI2, and SPMI-HIF2-1 (5, 20, or 50 µM) for 48 h. Apoptosis was examined with an Annexin V-FITC Apoptosis Detection Kit. The cell cycle distribution and apoptosis were detected by flow cytometry. Data are shown as the mean ± SEM of three independent experiments. P-values were calculated using the t-test (*P < 0.05).