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. 2022 Sep 14;13:961085. doi: 10.3389/fpls.2022.961085

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Copyright © 2022 Singh and Wesemael.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Diagram showing the nematode parasitism experimental setup and timeline for susceptibility test on seedlings. Tomato seedlings of 3 weeks old were soil-drenched with bacterial suspension (BS) at doses of 30, 50, and 100%, while control plants were mock- and buffer-treated (Ctrl and BF) with the positive control, β-aminobutyric acid (BABA). Four weeks after germination (1 week after application of the bacteria), plants were inoculated with 300 M. incognita J2 per plant and were analyzed for the total number of galls and egg laying females (ELF) at 42 days post-inoculation (dpi), the number of J2 and J3/J4 stages at 2 and 7 dpi. This figure was created with www.BioRender.com.