Figure 4.

IFNλ4 is expressed in macrophages and neutrophils in granulomas. (A) A representative lung granuloma stained with H&E (top left) to indicate the lymphocyte cuff (LC), epithelioid macrophage (EM), and caseous (C) regions and to detect IFNλ4 (red), CD11c+ macrophages (blue), and calprotectin+ neutrophils (green) (bottom left). Nuclei were stained with DAPI (grey). The white boxes in the immunofluorescence image indicate regions depicted in (B) Scale bar represents 500 μm. (B) Region 1 shows IFNλ4 (red) expression in CD11c+ macrophages (blue). Region 2 shows IFNλ4 (red) expression in calprotectin+ neutrophils (green). Images acquired at 20x magnification, scale bars represent 50 μm. (C) Percentage of CD11+ macrophages, calprotectin+ neutrophils, and other cells expressing IFNλ4 in granulomas (n=13). Median values for granuloma are shown where each marker color represents an animal. Statistical comparison by Tukey’s multiple comparisons test. (D) IFNλ4 expression by CD11c+ macrophages and calprotectin+ neutrophils as measured by median fluorescence intensity by cell subset per granuloma (n=13 granulomas). Each point depicts the median values for macrophages or neutrophils per granuloma, with each marker’s color representing a different animal. Statistical comparison by Wilcoxon matched-pairs signed rank test.