Fig. 1.

Representative ITC data for the binding of the high affinity ligands, cocaethylene (CE), cocaine (Coc), and benzoylecgonine (BE) to h2E2 mAb at 20°C in PBS buffer, pH = 7.4. The baseline-leveled raw ITC data is the upper panel, and the fitting to the resultant titration curve using a single binding site model is the lower panel for each ligand. The concentrations of ligands in the syringe and h2E2 mAb in the sample cell are given in the top panel, and the fitted parameters describing the binding for each ligand are given in the bottom panels.