Fig. 2.

ITC data for the binding of norcocaine (NC) to the intact h2E2 mAb at 20°C in PBS buffer, pH = 7.4. The concentrations of NC in the titration syringe and the concentration of the h2E2 mAb in the ITC cell were varied to generate fitted curves covering varying portions of the total binding curve. The baseline-leveled raw ITC data is the upper panel, and the fitting to the resultant titration curve using a single binding site model is the lower panel for each condition. The concentrations of ligands in the syringe and h2E2 mAb in the sample cell are given in the top panel, and the fitted parameters describing the binding for each condition are given in the bottom panels.