Table 3.
Primer sequences for RT-qPCR of human.
| Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) |
|---|---|---|
| ACSS1 | CGAGAGCGTTGCTTTGATCT | GGGCATGTAGATGGCAACAC |
| PCCA | CGTGGAGTTCCTTGTGGACT | CAGCTTGTTTGTGCCTGAGA |
| MCEE | GGAGCACATGGAAAACCAGT | TGGAAGCAGTGAAGGACTCA |
| MMUT | GAGTGGAGCATATCGCCAGG | CACTTCACGAGGAGTCTGGAA |
| SUCLG2 | TCACAGCTGATCCTAAGGTTG | GCGACGTTCTCTTGGATACC |
| FBP1 | CCTGCCGTCACTGAGTACAT | CAGCAGTCTCAGCTTTCCAT |
| FBP2 | AAGAAATTCCCTGAGGATGGCAG | GCCACGGGATTGCATTCATAC |
| PCK1 | CCTGACCGCAGAGAGATCAT | CCGCCAGGTACTTCTTCTCA |
| PCK2 | CCACTGGCATTCGAGATTTT | CCCGCTGAGAAGGAGTTACA |
| GAPDH | CAACGAATTTGGCTACAGCA | AGGGGTCTACATGGCAACTG |
ACSS1 = acyl-CoA synthetase short-chain family member 1; PCCA = propionyl-CoA carboxylase alpha chain; MCEE = methylmalonyl-CoA epimerase; MMUT = methylmalonyl-CoA mutase; SUCLG2 = succinate-CoA ligase; FBP = fructose 1,6-bisphosphatase; PCK = phosphoenolpyruvate carboxykinase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.