TABLE 6.
Results of Targeted Sequencing in a Subset of 32 Margin Positive Patients
| Case No. | IPMN | Margin | Relatedness | Recurrence (Type) |
|---|---|---|---|---|
| 1 |
KRAS: G12D (39.0) GNAS: R201C (27.9) |
KRAS: G12D (20.5) GNAS: wt |
Potentially related | PDAC |
| 2 |
KRAS: G12V (17.0), G12D (11.0), G12C (3.0) GNAS: wt |
KRAS: G12V (12.7), G13D (6.3) GNAS: wt |
Potentially related | IPMN |
| 3 |
KRAS: G12V (29.7) GNAS: R201C (23.4), R201H (1.9) |
KRAS: G12R (23.1), Q61H (18.1) GNAS: R201H (17.0), R201C (14.0) |
Potentially related | IPMN |
| 4 | KRAS: G12V (26.8) | wt | Independent | Progression on imaging |
| 5 |
KRAS: G12D (45.2), Q61H (5.0) GNAS: R201C (27.8) RNF43: wt |
KRAS: G12D (13.5), G12R (9.1) GNAS: R201C (7.8) RNF43: A136D (12.8) |
Potentially related | Progression on imaging |
| 6 |
KRAS: G12D (35.0), G12V (6.0) GNAS: R201H (2.6) |
KRAS: G12D (27.8) GNAS: wt |
Potentially related | Progression on imaging |
| 7 |
KRAS: G12D (32.0) GNAS: R201H (5.8) |
KRAS: G12V (13.8), G12D (6.5), G12R (18.8) GNAS: R201H (4.5) | Potentially related | Progression on imaging |
| 8 |
KRAS: Q61H (8.3), G12D (5.0) GNAS: R201H (3.1) |
KRAS: G12R (9.5) GNAS: wt |
Independent | Progression on imaging |
| 9 | KRAS: G12D (44.0) | KRAS: G12D (25.6) | Potentially related | Progression on imaging |
| 10 | KRAS: G12D (18.1), G12V (10.0), G12R (3.0) | KRAS: G12D (25.5), G12R (6.8) | Potentially related | — |
| 11 |
KRAS: G12V (8.9), Q61H (28.3) GNAS: R201H (19.9) |
KRAS: G12V (59.7) GNAS: R201H (37.5) |
Potentially related | — |
| 12 |
KRAS: G12D (5.9) GNAS: R201H (30.2) RNF43: R531H (6.4) |
KRAS: G12D (37.5) GNAS: wt RNF43: wt |
Potentially related | |
| 13 |
KRAS: Q61H (48.5) GNAS: R201H (50.2) |
KRAS: G12C (10.0) GNAS: wt |
Independent | — |
| 14 |
KRAS: G12V (33.2) GNAS: R201H (29.4), R201C (6.1) RNF43: G741E (6.4) |
KRAS: G12V (29.7), G12D (4.7), G12R (6.0), G12S (6.0)GNAS: wt RNF43: wt |
Potentially related | |
| 15 | KRAS: G12D (4.0), G12R (6.0), G12C (11.0) | KRAS: G12R (10.0) | Potentially related | — |
| 16 | KRAS: G12D (22.6) | KRAS: G12D (39.8) | Potentially related | — |
| 17 |
KRAS: G12V (5.9), G12R (3.0) GNAS: R201H (22.6), R201C (4.8) |
wt | Independent | — |
| 18 |
KRAS: G12D (30.3), G12V (9.3), Q61H (7.3) GNAS: R201H (6.8), R201C (6.0) |
KRAS: G12D (50.0) GNAS: - | Potentially related | |
| 19 | KRAS: G12D (16.7), G12V (29.1) | KRAS: G12D (3.5), Q61H (31.8) | Potentially related | — |
| 20 |
KRAS: G13D (36.1), G12V (6.0), Q61H (14.3) GNAS: R201H (15.3) RNF43: wt |
KRAS: G12R (35.3), G12C (5.4) GNAS: wt RNF43: A46G |
Independent | |
| 21 | KRAS: G12V (28.0) | KRAS: G12D (17.0), G12C (7.5) | Independent | — |
| 22 |
KRAS: G13D (2.0) GNAS: R201H (36.0) |
KRAS: G13D (14.0), G12R (14.0) GNAS: wt |
Potentially related | — |
| 23 | GNAS: R201H (30.0) | wt | Independent | — |
| 24 |
KRAS: G12D (39.0) GNAS: R201C (32.5), R201H (6.5) |
KRAS: G12D (27.0), G12V (13.0) GNAS: R201C (22.5), R201H (7.0) |
Potentially related | — |
| 25 |
KRAS: G12D (9.1), G12V (10.4) GNAS: R201C (11.3) |
KRAS: G12D (14.6), G12V (10.4), G12R (10.4) GNAS: | Potentially related | — |
| 26 | GNAS: R201H (28.0) | wt | Independent | — |
| 27 |
KRAS: G12V (42.2), G12R (3.5) GNAS: R201C (16.5) |
KRAS: G12D (26.0), G12R (6.0) GNAS: wt |
Potentially related | — |
| 28 |
KRAS: G12R (41.1), G12D (6.0) GNAS: R201H (6.4) |
KRAS: G12D (30.3) GNAS: R201H (2.8) |
Potentially related | — |
| 29 |
KRAS: G12C (6.0), G12V (13.0) GNAS: R201C (6.6) |
KRAS: G12C (18.4), G12D (6.8), G12V (3.4), Q61H (13.8) GNAS: R201C (3.0) |
Potentially related | — |
| 30 |
KRAS: G13D (33.3), G12D (5.0) GNAS: R201H (19.5) |
KRAS: G12V (4.0) GNAS: wt |
Independent | — |
| 31 |
KRAS: G12D (37.2), G12V (5.0) GNAS: R201H (39.3) |
KRAS: G12D (7.0), G12R (24.5) GNAS: R201H (3.5) |
Potentially related | — |
| 32 | KRAS: G12D (31.4), G12V (26.6) | KRAS: G12D (45.0) | Potentially related | — |
Somatic mutations in driver genes in IPMNs and at the corresponding margins were analyzed and their relatedness assessed. Wt indicates wild type.