Fig. 6.
Effects of GPX4 overexpression on H2O2-induced apoptosis in the pancreatic acinar cells of Se-adequate chicks.
(A) Illustration of the control and GPX4 (OE) transfection into pancreatic acinar cells. a, bright filed of acinar cells (control without any transfection); b, 48 h after transfection of GPX4 (OE), fluorescence signal of GPX4 (green) in acinar cells. Images were taken with 800 × magnification. The image was a representative of five independent examinations.
(B) Effects of GPX4 overexpression on relative mRNA abundances (upper panel) and protein levels (lower panel) of GPX4 in the pancreatic acinar cells at different time points. Data are means ± SE, n = 5.
(C) Effect of GPX4 overexpression (48 h after transfection) on viability of the pancreatic acinar cells treated with 0 μM, 50 μM, or 100 μM H2O2 for 6 h. Data are means ± SE, n = 6.
(D) Effect of GPX4 overexpression (48 h after transfection) on protein levels of GPX4 and apoptotis signal proteins in the pancreatic acinar cells treated with 50 μM H2O2 for 6 h. Data are means ± SE, n = 3.
In B, C, and D, values within a given time, dose of H2O2, or protein differ (P < 0.05) without sharing a common superscript letter.
Abbreviations: ACTB, beta actin; cleaved-CASP 3, cleaved caspase 3; cleaved-CASP 9, cleaved caspase 9; Control, lysates of pancreatic acinar cells without transfection; ET, lysates of pancreatic acinar cells transfected with Entranster-H DNA transfection reagent only; CYTC, Cytochrome c; GPX4, glutathione peroxidase 4; ET + Plasmid, lysates of pancreatic acinar cells both transfected with pcDNA3.1/GPX4-EGFP and Entranster-H DNA transfection reagent; Plasmid, lysates of pancreatic acinar cells transfected with pcDNA3.1/GPX4-EGFP only; pro-CASP 3, pro caspase 3; pro-CASP 9, pro caspase 9.