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. 2022 Sep 17;57:102482. doi: 10.1016/j.redox.2022.102482

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 8 — Effects of the shGPX4 transfection on chicken embryo of the EGFP expression, pancreatic histology, GPX4 mRNA expression, production of pancreatic apoptotic signal proteins, and hatch rate.

(A) Expression of EGFP of transgenic chicken embryo (day 8). shGPX4, eggs injected with pLL3.7-GPX4-shRNA; control, eggs injected with empty vector.

(B) H&E staining of paraffin-embedded chicken embryonic pancreas (day 18) sections. Compared with the control, the shGPX4 chicken embryonic pancreas showed acinar cell swelling, granular degeneration and vacuolar degeneration. Images were taken with 400 × magnification. The image was a representative of five independent examinations.

(C) Effects of the shGPX4 transfection on relative mRNA abundances of GPX4 in the day18 embryonic pancreas. Data are means ± SE, n = 5, *P < 0.05 compared with the control.

(D) Effects of the shGPX4 transfection on protein levels of GPX4 and apoptotic signal proteins in the day18 embryonic pancreas. Data are means ± SE, n = 5, *P < 0.05 compared with the control.

(E) Effects of shGPX4 and shGPX1 transfections on egg hatching rates. GPX1, eggs injected with pLL3.7-GPX1-shRNA; shGPX4, eggs injected with pLL3.7-GPX4-shRNA; Control, eggs injected with empty vector.

Abbreviations: ACTB, beta actin; cleaved-CASP 3, cleaved caspase 3; cleaved-CASP 9, cleaved caspase 9; Control, eggs injected with the empty vector; CYTC, Cytochrome c; GPX1, glutathione peroxidase 1; GPX4, glutathione peroxidase 4; pro-CASP 3, pro caspase 3; pro-CASP 9, pro caspase 9; shGPX1, chicken eggs injected with pLL3.7-GPX1-shRNA; shGPX4, chicken eggs injected with pLL3.7-GPX4-shRNA.