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. Author manuscript; available in PMC: 2022 Oct 13.
Published in final edited form as: Sci Transl Med. 2022 Apr 13;14(640):eabk1107. doi: 10.1126/scitranslmed.abk1107

Fig. 4. In vivo competition between B. infantis strains in gnotobiotic mice.

Fig. 4.

(A) Shown is the experimental design of the study examining the fitness of five B. infantis strains in gnotobiotic mice consuming the Mirpur-6 diet ± LNT or LNnT. (B-D) Shown are results of competition experiments involving the 5-member consortium of B. infantis strains introduced into gnotobiotic mice. (E) The same 5-member B. infantis consortium shown in panels B-D was introduced together with a B. bifidum strain isolated from a healthy 12-month-old Bangladeshi child into gnotobiotic mice. Absolute abundances of the different strains, as a function of time and HMO supplementation, were determined by short read shotgun sequencing of fecal DNA. Mean values ± SD are plotted; n=6-7 mice per group; n=1 experiment. Timepoints where the absolute abundance of the B. infantis strain Bg_2D9 was higher than other consortium members were identified using a mixed effects linear model followed by Tukey’s multiple comparison test. *, Padj<0.05.