Figure 4.

Impact of the AQ series on the formation of metal-free or metal-treated Aβ₄₀ aggregates with and without light activation. (a) Scheme of the inhibition experiments. (b) Gel/Western blots (anti-Aβ antibody, 6E10) of the resultant Aβ₄₀ species upon incubation of metal-free and metal-added Aβ₄₀ with and without treatment of compounds and light. Lanes: (C) Aβ₄₀; (1) Aβ₄₀ + 9,10-AQ; (2) Aβ₄₀ + HQ; (3) Aβ₄₀ + Ali; (4) Aβ₄₀ + Qui; (5) Aβ₄₀ + Pur; (6) Aβ₄₀ + Dan; (7) Aβ₄₀ + Rhe; (8) Aβ₄₀ + Alo. (c) TEM images of the aggregates produced upon 24 h incubation of metal-free and metal-treated Aβ₄₀ with and without the AQ series in the absence and presence of light. Conditions: [Aβ₄₀] = 25 μM; [M(II)] = 25 μM; [compound] = 50 μM; 20 mM HEPES (1% v/v DMSO), pH 7.4 [for metal-free or Zn(II)-containing samples] or pH 6.6 [for Cu(II)-added samples], 150 mM NaCl; 37 °C; 24 h; constant agitation (250 rpm); Kessil lamp (467 nm) for 1 h. Scale bar = 200 nm.