Figure 5.

Detection of the Aβ₄₂ aggregate deposition in murine brains injected with photoactivated Alo-treated Aβ₄₂ species. (a) Schematic description of histochemical studies and injection sites in the brain. Hippocampus (hip), cortex (ctx), thalamus (th), caudate putamen (cp), and hypothalamus (hy) are shown. (b) Microscopic images of the hippocampi of C57BL/6J mice injected with vehicle, Aβ₄₂, or Alo-treated Aβ₄₂. Aβ species were prepared in 20 mM HEPES, pH 7.4, 150 mM NaCl (1% v/v DMSO; vehicle) and visualized by immunostaining [primary antibodies, 6E10 (anti-Aβ antibody; yellow) and A11 (antioligomer antibody; red) with fluorescent-conjugated secondary antibodies] or fluorescent dyes [ThS for Aβ fibrils (green) and DAPI for fibrillary Aβ and nucleus (blue)]. Images were taken by a confocal microscope (left; ×10) or a scanning microscope (right; ×10). Detected amyloid species are marked with white arrows. Scale bars = 250 and 500 μm for confocal or scanning microscopies, respectively. Animal number: n = 4 (for Aβ₄₂ and Alo-added Aβ₄₂) and n = 3 (for vehicle).