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. 2022 Feb 22;190(2):1024–1036. doi: 10.1093/plphys/kiac072

Figure 2.

Figure 2

Light has a competitive effect on the sub-nuclear localization of ELF3. Hypocotyl nuclei of seedlings expressing 35S::YFP:ELF3 under different light treatments at ZT10 (short-day 8/16 photoperiods). A, Darkness; B, 25 µmol monochromatic RL; C, WL: 25 µmol of RL and 12 µmol of BL; D, WL: 25 µmol of RL and 25 µmol of BL; E, WL: 20 µmol of RL and 40 µmol of BL; and F, 25 µmol monochromatic BL. Scale bars are 5 µm. All light pulses were applied for 3 h starting at ZT7. G, Mean number of foci per nucleus under the respective light treatments. Error bars are standard error of the mean. A minimum of 11 nuclei were imaged for each light treatment with images collected from multiple biological samples. Significance was determined by a one-way ANOVA with a Tukey HSD post hoc test. Different letters signify significant difference of P < 0.001. The darkness, monochromatic BL, and RL datasets are the same as presented in Figure 1.