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. 2022 Feb 22;190(2):1024–1036. doi: 10.1093/plphys/kiac072

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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phyB is required for ELF3 to localize to foci. The localization of 35S::YFP:ELF3 in hypocotyl nuclei of either the (A–B) elf3-4 or (C–D) elf3-4/phyB-10 mutant. Samples were either transferred to the (A, C) dark or (B, D) pulsed with 25 µmol RL pulse for 3 h at ZT7 before images were collected at ZT10. Scale bars represent 5 µm. E, Mean number of foci per nucleus for the respective construct and light treatment. A minimum of 12 images were analyzed for each light treatment. Error bars are standard error of the mean. Significance was determined by a one-way ANOVA with a Tukey HSD post hoc test to determine significance. Different letters signify a significant difference of P < 0.001. The values for 35S::YFP:ELF3 (elf3-4) are the same as first presented in Figure 1.