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. 2022 Feb 22;190(2):1024–1036. doi: 10.1093/plphys/kiac072

Figure 6.

Figure 6

Complex effect of phyB in regulating ELF3 transcriptional activity. The expression of (A) PRR9 (B) GI (C) TIMING OF CAB1 EXPRESSION (TOC1), and (D) LUX ARRYTHMO (LUX) was measured in WT (Ws-2), 35S::YFP:ELF3 elf3-4 (ELF3ox B+), or 35S::YFP:ELF3 elf3-4/phyB-10 (ELF3ox B-) at ZT10. Respective lines were either prior pulsed for 3 h with 25 µmol of RL (red bars) or transferred to the dark (gray bars) at ZT7 as a control. Data were normalized to the expression of PP2A. The presented data are the mean of at least two technical replicas. Error bars indicate standard deviation among the technical replicas. These results are representative of two biological replicas. E, The relative change in the expression of PRR9, GI, TOC1, and LUX following a RL pulse compared to the expression of the respective gene in the dark at ZT10.