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. 2022 May 4;190(2):1057–1073. doi: 10.1093/plphys/kiac196

Figure 3.

Figure 3

Identification of genome-wide direct targets of OsCCA1. A, The number of bound genes by OsCCA1 obtained by DAP-seq with P < 0.05 and their distribution in the whole genome. The promoter regions were determined as the binding peaks within 3,000-bp upstream of ATG. B, The identified binding motifs of OsCCA1 protein by MEME-ChIP. The core sequence of “AAAATATCT” was substantially enriched among the OsCCA1 binding regions (e-value = 6.3e-1202) and was named as rCBS. C and D, Results of EMSAs confirming OsCCA1 bound to the AAATATC motif of SRZ1pro and OsPP2C09pro. The unlabeled probe was taken as competitor. GST alone was used as negative control of the binding. Red arrowhead indicates the DNA–protein complex. E, The top KEGG-enriched terms of OsCCA1-bound genes by DAP-seq.