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. 2022 Jun 25;190(2):1334–1348. doi: 10.1093/plphys/kiac312

Figure 1.

Figure 1

Generation of tomato Δnrd1 mutants by CRISPR/Cas9. A, Schematics showing the guide-RNA (gRNA) target site and the missense mutations present in two independent Δnrd1 lines. The Δnrd1-1 line has a 2-bp deletion and the Δnrd1-2 line has a 13-bp deletion. Wild-type is Rio Grande (RG)-PtoR. UTR, untranslated region. B, Photographs of 4-week-old wild-type RG-PtoR and the two Δnrd1 mutant lines grown in the greenhouse. C, Phylogenetic tree of Nrd1 and related proteins. Amino acid sequences of Nrd1 and related proteins in Arabidopsis (blue squares), rice (green circles), and tomato (red triangles) were used to generate a maximum likelihood tree. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Numbers on branches indicate bootstrap support of the nodes (%). The red arrow indicates the Nrd1 protein. The asterisks indicate genes that have been reported to be implicated in immunity (Huibers et al., 2009; Wang et al., 2015b; Schwartz et al., 2017; Kim and Mudgett, 2019).