Figure 2.

Investigation of ETI- and PTI-mediated immunity in the Δnrd1 mutants. Four-week-old Δnrd1 plants, Rio Grande (RG)-PtoR (wild-type), and RG-prf3 (a Prf mutant) plants were vacuum-infiltrated with: A, 1 × 10⁶ cfu · mL⁻¹ DC3000 or B, 5 × 10⁴ cfu · mL⁻¹ DC3000ΔavrPtoΔavrPtoB (DC3000ΔΔ). Photographs of disease symptoms were taken at 6 days (A) or 5 days (B) after inoculation. Bacterial populations were measured at 3 h (Day 0) and 2 days (Day 2) after infiltration. Cfu, colony-forming unit. Bars show means ± standard deviation (sd). Different letters indicate significant differences based on a one-way analysis of variance (ANOVA) followed by Student’s t test (P < 0.05). ns, no significant difference. Three plants for each genotype were tested per experiment. The experiment was performed three times with similar results.