Figure 4.

Transient overexpression of candidate immunity-related proteins in N. benthamiana leaves followed by a bacterial pathogenicity assay. A and B, Leaves of 5-week-old N. benthamiana plants were syringe-infiltrated with Agrobacterium (1D1249) strains (Optical Density (OD) = 0.5) carrying a binary expression vector expressing each gene. Two days later, the same agroinfiltrated spots were syringe-infiltrated with 5 × 10⁴ cfu · mL⁻¹ DC3000ΔhopQ1-1 (A) or 5 × 10⁴ cfu · mL⁻¹ DC3000ΔhopQ1-1ΔavrPtoΔavrPtoB (B). Pst DC3000 populations were measured 2 days after the second infiltration. Cfu, colony-forming unit. Bars show means ± sd. Different letters indicate significant differences based on a one-way ANOVA followed by Student’s t test (P < 0.05). ns, no significant difference. Three (A) or four plants (B) were tested with each gene in each experiment. Each experiment was performed at least two times with similar results.