Figure 6.

Analysis of Ca²⁺-dependent interaction of MsCML10 with MsGSTU8 or MsFBA6. Nicotiana benthamiana leaves co-transformed using the mixed Agrobacterium harboring 35S::cLUC-MsCML10 and 35S::MsGSTU8-nLUC or 35S::MsFBA6-nLUC were placed on 4% agar containing 10- or 20-mM CaCl₂, 20-mM EGTA or without any supplement as control for 48 h, followed by capturing the luciferase image (A) or measuring the fluorescence intensity as the relative LUC activity (B). Means of the data from eight-leaf discs and standard errors are presented; the same letter above the column indicates no significant difference at P < 0.05 using Duncan’s test.