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. 2022 Sep 27;23:204. doi: 10.1186/s13059-022-02765-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Amount and characteristics of missing genes and exons. a GC and repeat content of completely missing genes in previous assemblies (red) but present in the VGP assemblies compared to those of genes present (gray) within both previous VGP assemblies. b Percent missing of exonic, intronic, non-coding genic, and intergenic sequences in the prior assemblies. c Cumulative density plot of protein-coding genes as a function of percent missing sequence. Illumina-based assemblies (Anna’s hummingbird and climbing perch) have more complete genes compared to Sanger-based assemblies (zebra finch and platypus). Gray dashed line indicates where 10% of a gene is missing