TABLE 3.

Partner site preferences of 59-be cloned in orientation 2 in pACYC184

Plasmid	59-be	Fragment lengtha	Cointegration frequency			% Tpsb (range)	No. mappedc	R388 recombination site used
			No. of assays	Range	Mean
pRMH553	aadB/qacE	154/198	9	2.6 × 10−3–1.0 × 10−2	8.4 × 10−3	88 (84–91)	17	15 attI1; 2 orfA 59-be
			6	2.4 × 10−4–1.8 × 10−3	9.6 × 10−4	38 (32–45)	13	6 attI1; 5 orfA 59-be; 2 2°rs
			4	3.8 × 10−3–2.4 × 10−2	1.1 × 10−2	99 (98–99)	12	12 attI1
pRMH643	orfA/qacE	109/98	9	7.3 × 10−5–2.0 × 10−3	5.1 × 10−4	48 (27–66)	23	14 attI1; 5 dfrB2 59-be; 4 orfA 59-be
			4	1.8 × 10−4–9.9 × 10−3	3.6 × 10−3	85 (78–90)	24	20 attI1; 3 orfA 5-be; 1 2°rs
pRMH800	aadA1/qacE	197/198	8	2.0 × 10−3–1.6 × 10−2	6.9 × 10−3	63 (45–79)	32	19 attI1; 4 dfrB2 59-be; 4 orfA 59-be; 5 2°rs
pMAQ542	aadB/oxa2	120/83	6	2.0 × 10−4–3.7 × 10−3	1.7 × 10−3	NA	18	5 attI1; 4 dfrB2 59-be; 9 orfA 59-be
pRMH556	dfrA7/qacE	221/755	9	2.8 × 10−4–9.2 × 10−4	6.3 × 10−4	NA	16	15 attI1; 1 orfA 59-be
			6	5.4 × 10−4–2.9 × 10−3	1.3 × 10−3		29	23 attI1; 3 orfA 59-be; 3 2°rs

^aNumbers refer to nucleotides in the cloned fragment to the left and right of the recombination crossover point. 

^bAverage with range of values from individual crosses shown in parentheses. NA, not applicable 

^cRandomly selected recombinants were mapped.