TABLE 4.

Effect of 59-be site orientation on recombination with attI1

Plasmida	Recombination site	Orientation	Mean cointegration frequencyb
			R388c	pRMH560	pRMH821d
pMAQ28	aadB/qacE 59-be	1	1.1 × 10−2 (5)	1.2 × 10−7 (7)	5.3 × 10−2 (4)
pRMH553	aadB/qacE 59-be	2	9.2 × 10−3 (13)	6.3 × 10−2 (6)	4.4 × 10−2 (4)
pRMH642	orfA/qacE 59-be	1	2.4 × 10−3 (10)	5.5 × 10−6 (6)	ND
pRMH643	orfA/qacE 59-be	2	1.5 × 10−3 (13)	3.6 × 10−3 (4)	ND
pRMH251	attI1/qacE	1	7.3 × 10−3 (14)	4.3 × 10−8 (11)	ND
pRMH313	attI1/qacE	2	3.5 × 10−3 (9)	2.5 × 10−5 (6)	ND
pACYC184			1.6 × 10−5 (13)	5.4 × 10−7 (14)	ND

^aAll strains also contain pSU2056. 

^bNumber of determinations is shown in parentheses. ND, not determined. 

^cSome values were previously given in Tables 2 and 3. 

^dP_c (weak) sequence: −35 TGGACA, −10 TAAGCT.