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Northern blot analysis of the sat operon. Ten micrograms of total RNA from pH 7- or 5-shocked S. mutans NG8 cells was hybridized with an ffh probe amplified with primers PC18 and PC20 (A), a satE probe amplified with primers BK14 and BK15 (B), and an opuABC probe amplified with primers BK5 and BK6 (C). Arrows indicate major mRNA transcripts. RNA size markers are indicated to the left of panel A.
