TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMA-mcrBC) φ80lacZΔM15 ΔlacZ74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| INV110 | F−traD36 proAB+ lacIqZΔM15/rpsL (Strr) thr leu endA thi-1 lacYgalK galTara tonA tsx dam dcm supE44 Δ(lac-proAB) Δ(mcrC-mrr) 102::Tn10 (Tetr) | Invitrogen |
| INVαF′ | F−endA1 recA1hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 | Invitrogen |
| S. mutans | ||
| NG8 | Wild type | K. Knox |
| MK4 | ffh::Kanr; pMK14 in NG8 | This study |
| BK110 | satC::Kanr; pBK40 in NG8 | This study |
| BK120 | satD::Kanr; pBK41 in NG8 | This study |
| BK130 | satE::Kanr; pBK42 in NG8 | This study |
| BK140 | ΔylxM::Kanr; pBK45 in NG8 | This study |
| BK150 | gtfA::ylxM-ffh; pBK55 in NG8 | This study |
| BK151 | ffh::KanrgtfA::ylxM-ffh; pMK14 in BK150 | This study |
| BK152 | gtfA::pVA891; pVAgtfA in NG8 | This study |
| Plasmids | ||
| pALH124 | aphA3 Kanr cassette in pALH123 | A. L. Honeyman |
| pBAD-TOPO | Arabinose-inducible expression of His6 tag protein | Invitrogen |
| pCR2.1 | PCR product A/T cloning vector | Invitrogen |
| pPC185 | pUC18 derivative; Emr Apr | This study |
| pPC300 | Psat-ylxM-ffh in pDL278 | 11 |
| PUC18 | E. coli cloning vector | Invitrogen |
| pVA891 | Streptococcal suicide vector | 30 |
| pVAGTFA | gtfA in pVA891 | 26 |
| pBK8 | ffh in pBAD-TOPO | This study |
| pBK19 | Psat-ylxM in pCR2.1 | This study |
| pBK22 | satD in pCR2.1 | This study |
| pBK23 | satE in pCR2.1 | This study |
| pBK40 | satC::Kanr in pPC185 | This study |
| pBK41 | satD::Kanr in pPC185 | This study |
| pBK42 | satE::Kanr in pPC185 | This study |
| pBK43 | 5′ ylxM in pCR2.1 | This study |
| pBK44 | 3′ ylxM in pCR2.1 | This study |
| pBK45 | ΔylxM::Kanr in pUC18 | This study |
| pBK55 | Psat-ylxM-ffh (pPC300-derived) in pVAGTFA | This study |
| pMK2 | satD in pPC185 | This study |
| pMK4 | satC in pCR2.1 | This study |
| pMK6 | satE in pPC185 | This study |
| pMK8 | satC in pPC185 | This study |
| pMK10 | ffh in pCR2.1 | This study |
| pMK11 | ffh in pPC185 | This study |
| pMK14 | ffh::Kanr in pPC185 | This study |