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. 2001 Apr;183(8):2576–2585. doi: 10.1128/JB.183.8.2576-2585.2001

FIG. 6.

FIG. 6

RNase protection of a 150-nucleotide riboprobe for rhtA and a 100-nucleotide riboprobe for rhbA. Lane 1, RNA from mutant 2011 rhrA26 grown under iron-depleted conditions; lane 2, RNA from 2011::Tn5 grown under iron-depleted conditions; lane 3, RNA from 2011::Tn5 grown under iron-replete conditions. The Tn5 insertion in 2011::Tn5 does not affect rhizobactin 1021 regulation, biosynthesis, or transport. A 200-nucleotide riboprobe for the npt gene of Tn5 allows comparison of RNA levels in the reactions.