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Products from RT-PCR separated on a 4% agarose gel. The RNA template for the reactions in lanes 1, 2, and 3 was from free-living cells grown under iron-depleted conditions. The RNA template for the reactions in lanes 4, 5, and 6 was from nodules. Lanes 1 and 4, reactions primed for a 400-nucleotide nifH fragment; lanes 2 and 5; reactions primed for a 200-nucleotide npt fragment; lanes 3 and 6; reactions primed for a 148-nucleotide rhbF fragment.
