TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmida | Relevant characteristic(s)b | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| S605 | met::Tn5 | W. Klipp, Bielefeld, Germany |
| A118 | Chromosomally located Tn5lac | 49 |
| DH5α | hsdR17 endA1 thi-1 gyrA96 relA1 recA1 supE44 ΔlacU169 (φ80dlacZΔM15) | Gibco-BRL |
| S. meliloti | ||
| 2011 | Wild typec | |
| 2011 rhbA62 | rhbA::Tn5lac, rif | This study |
| 2011 rhbC2 | rhbC::Tn5mob, rif | 46 |
| 2011 rhbE11 | rhbE::Tn5lac, rif | This study |
| 2011 rhbE9 | rhbE::Tn5, rif | This study |
| 2011 rhbF104 | rhbF::Tn5lac, rif | This study |
| 2011 rhbF107 | rhbF::Tn5lac, rif | This study |
| 2011 rhrA4 | rhrA::Tn5lac, rif | This study |
| 2011 rhrA6 | rhrA::Tn5, rif | This study |
| 2011 rhrA26 | rhrA::Tn5lac, rif | This study |
| 2011 rhtA45 | rhtA::Tn5lac, rif | This study |
| 2011 rhtA42 | rhtA::Tn5lac, rif | This study |
| 2011 rhtA1 | rhtA::Tn5, rif | This study |
| SmA818 | Cured of pSyma | 40 |
| SmA416BTW | Deletion in pSyma | 40 |
| Plasmids | ||
| pUC18 | Apr, lacZ′ | 36 |
| pJQ200ks | Gmr, mob sacB | 45 |
| pGR30 | Cosmid carrying rhizobactin 1021 synthesis, regulation, and uptake genes, Tcr | 46 |
| pRK600 | Tra+, Cmr | 15 |
| pDL80 | pJQ200ks carrying 8.0-kb genomic BamHI insert | This study |
| pUC18–52 | pUC18 carrying 5.2-kb genomic BamHI insert | This study |
| pDL30 | pJQ200ks carrying 3.0-kb XhoI-BamHI fragment from pUC18–52 | This study |
a
pUC18-based constructs used for sequencing are not included.
b
Apr, Cmr, Gmr, and Tcr, ampicillin, chloramphenicol, gentamicin, and tetracycline resistance, respectively; mob, mobilization; Tra+, transfer.
c
S. meliloti 2011 is the parent strain of 1021 (35) from which rhizobactin 1021 was purified and chemically characterized (42). A rifampin-resistant derivative (rif) was used in the construction of transposon insertion mutants. To facilitate the use of the npt probe to compare relative abundance of RNA in Tn5lac-induced mutants and wild type, a derivative with a Tn5 insertion in a rhamnose utilization gene (provided by Michael Hynes, University of Calgary) was used instead of wild type.