FIG 6.

TGEV infection promoted the self-renewal of porcine intestinal stem cells via the Wnt/β-catenin pathway. (A) The optimal concentration and treated time of ICG-0001 were screened based on the expression of β-catenin, C-myc, and Sox9. (B) The density ratios of β-catenin, C-myc, and Sox9 were calculated with Image J and normalized against GAPDH expression. ns, not significant; **, P ≤ 0.01; ***, P ≤ 0.001. (C) The expression of C-myc and Sox9 were detected in organoid monolayer treated with 10 μM ICG-001 before TGEV infection. (D) The density ratios of C-myc and Sox9 were calculated with Image J and normalized against GAPDH expression. ns, not significant; **, P ≤ 0.01; ***, P ≤ 0.001.