FIG 4.

Avatar mice recapitulate EVD but not Lassa fever. (A) Model schematic. EBOV, Ebola virus; RESTV, Reston virus; and LASV, Lassa virus. (B) Kaplan-Meier survival curves of avatar mice transplanted with EBOV-infected moDCs (red), RESTV-infected moDCs (blue), or LASV-infected DCs (green). (C) Body weight loss curves of avatar mice infected with each one of the three viruses. (D) Levels of serum AST at the indicated time points in all three models. (E) Levels of viremia. The gray areas represent the limit of detection of the assay. (F) Virus titers in organs at the time of necropsy. Organ titers were calculated via immunofocus assay as described in Materials and Methods section. Across the figure, significance levels are presented as follows: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; and ****, P ≤ 0.001. The survival curves were assessed via Mantel-Cox, and the comparison of virus titers in organs was assessed via a Kruskal-Wallis nonparametric analysis. All other analyses were conducted with a two-way ANOVA followed by Dunnett’s multiple comparison test. Data are shown as mean ± SEM.