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ORF7 mC1H1 KSHV failed to cleave the KSHV TR appropriately. Southern blotting of the EcoRI- and SalI-digested genomic DNA purified from Dox- and NaB-treated iSLK cells harboring WT-KSHV (WT-iSLK), ORF7-KO-KSHV (ORF-fsKO-iSLK and ORF7-stKO-iSLK) as well as ORF7 mC1H1-KSHV (ORF7 mC1H1-iSLK). Digested DNA was detected with a DIG-labeled TR probe. Left panel, short exposure; right panel, long exposure.
