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. 2022 Sep 8;96(18):e00684-22. doi: 10.1128/jvi.00684-22

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Copyright © 2022 Iwaisako et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Construction of ORF7&17-DKO-KSHV, and TEM images of capsids produced from ORF7&17-DKO-KSHV. (A) Schematic illustration of the genetic locus of ORF17 and the mutation added to ORF7-fsKO-BAC16 for construction of ORF7&17-DKO-BAC16. The neighboring DNA sequence of the additional mutation in ORF7&17-DKO-BAC16 was confirmed by Sanger sequencing. (B and C) TEM images of capsids produced from WT-KSHV and ORF7&17-DKO-KSHV. iSLK cells harboring WT-KSHV (WT-iSLK) or ORF7&17-DKO-KSHV (ORF7&17-DKO-iSLK) were cultured for 72 h in medium containing Dox and NaB for induction of the lytic phase. Capsid formation in nuclei from (B) WT-iSLK cells and (C) ORF7&17-DKO-iSLK cells were observed by TEM. The black arrowheads, white arrowheads, black arrows, and white arrows indicate C-capsids, A-capsids, B-capsids, and soccer ball-like capsids, respectively. The scale bars represent a length of 100 nm. (D) Quantification of the number of each type of capsid observed by TEM.