FIG 5.

TR cleavage does not occur in both ORF7 and ORF17 DKO KSHV as well as ORF17 KO KSHV. (A) Southern blotting of the EcoRI- and SalI-digested genomic DNA purified from Dox- and NaB-treated iSLK cells harboring WT-KSHV (WT-iSLK), ORF7-KO-KSHVs (ORF7-fsKO-iSLK and ORF7-stKO-iSLK), ORF17-KO-KSHV (ORF17-KO-iSLK), and ORF7&17-DKO-KSHV (ORF7&17-DKO-iSLK). The digested DNA was detected with a DIG-labeled TR probe. Left panel, short exposure; right panel, long exposure. (B) Overview of the predictive KSHV capsid formation process, including ORF7, ORF17, and soccer ball-like capsids. The period of KSHV genome cleavage and genome insertion into capsids is represented by a bold line. MCP, major capsid protein; TRI, triplex; PORT, portal protein; SCAF, scaffold protein.