FIG 7.

Soluble huCD320 harboring L55 and W69 of Tva effectively blocked viral entry. (A and B) Entry of RCASBP(A) virus into DF-1 cells incubated with different soluble chimeric huCD320 or Tva proteins. Bovine serum albumin (BSA) was used as a negative control. (A) Virus entry levels as analyzed by fluorescence microscopy at 7 days postinfection (dpi). Scale bar: 125 μm. (B) Virus entry levels as analyzed by counting the proportion of GFP-positive cells using flow cytometry at 7 dpi. The entry level of RCASBP(A) virus incubated with BSA was set to 100% and the values for the soluble chimeric huCD320 and Tva proteins were calculated as their proportions. (C) Entry of RAV-1 virus into DF-1 cells incubated with the soluble chimeric huCD320 or Tva proteins as assessed by measuring virus titers in cell supernatants collected 7 dpi. BSA was used as a negative control. Data from three independent experiments are shown as means ± standard deviations of triplicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significant difference.