FIG 6.

T-cell cytokine response induced by bivalent VSV vaccine candidates. (A) Splenocytes isolated from immunized mice (as described in Fig. 4A) were cultured without peptide (no peptide control, NC) (a to c), or stimulated with SARS-CoV-2 SP subunit 1 (S1) peptide pool (d to f) or influenza M2e peptide (g to i) (1 μg/mL for each peptide). After 4 days of stimulation, supernatants were collected, and the release of IFN-γ, IL-4, and IL-5 cytokines in the supernatants was quantified with an MSD U-plex mouse cytokine immunoassay kit and counted in the MESO Quickplex SQ120 instrument. Each symbol indicated one individual mouse. (B) The ratios of Th1/Th2 cytokines from splenocytes of each mouse in the same culture condition were calculated, respectively, and the representative data (IFN-γ/IL-4) were shown. Statistical significance between the two groups was determined using an unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.