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Diverse flaviviruses replicate in the vagina of WT mice. Six-week-old wild-type mice pretreated with 2 mg of DMPA were inoculated with 1,000 FFU of ZIKV, 1,000 FFU of Spondweni virus (SPOV), 10,000 FFU of dengue virus (DENV-3), 1,000 FFU of Usutu virus (USUV), or 1,000 FFU of Kunjin virus (KUNV) by intravaginal instillation. Viral RNA was measured from vaginal washes by qRT-PCR.
