TABLE 1.

Primer sets used for qRT-PCR

Virus or gene target/primer type	Sequence
USUV (SA AR 1776)
Forward	TCACAACTAGGAGCATCACAAG
Reverse	CCATAGTCACCCATCTTCACAG
Probe	TTTACCATCACTCCCAACTCCCCAG
SPOV (SA AR 94)
Forward	TGTGCCAATGGTGGGTAAT
Reverse	GGAAAGTGGAGCAAACTCAAAG
Probe	CGAGATTGACCCTCCGTTTGGTGA
DENV3 (CH53489)
Forward	ATTACAGTGCACACAGGAGAC
Reverse	CTAGCCCAAGGGTTCCATATTC
Probe	TGGGAAATGAAACGCAGGGAGTCA
KUNV (FLSDX)
Forward	AGTGGATAGAGGTTGGGGCA
Reverse	AGTCCTTCCCGTTGCCTTG
Probe	ATCGACACATGCGCCAAATTTGCCTGT
RUBV (M33)
Forward	CGAACGAGAAGTGCGGTATATG
Reverse	GCGAAACGGCACTGAGAA
Probe	ACCTGCTCA/ZEN/ACAAGAATCACACCGA
HAV
Forward	GGTAGGCTACGGGTGAAAC
Reverse	AACAACTCACCAATATCCGC
Probe	AGATGCCTTGGATAGGGTAACAGCG
ActB
Forward	GACTCATCGTACTCCTGCTTG
Reverse	GATTACTGCTCTGGCTCCTAG
Probe	CTGGCCTCACTGTCCACCTTCC
Ifit1
Forward	TGAAGCAGATTCTCCATGACC
Reverse	GCAAGAGAGCAGAGAGTCAAG
Probe	ACAGCTACCACCTTTACAGCAACCAT