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TBEV replicons. (A) Schematic representation of the TBEV genome (top) and replicon system in which the structural genes were replaced with Spinach2, FMDV-2A, and firefly (cu) luciferase. WT Hypr and Vs replicons are shown as a reference for subsequent chimeric construction. (B) Black-and-white image of RNA-fluorophore complexes from SP6-transcribed TBEV RNA (1 μg) mixed with the Spinach substrate DFHBI for 30 min.
