FIG 8.

Hypr and Vs TBEV strains differentially induce caspases 3 and 8. (A) Immunofluorescence analysis of caspase-3 and -8 in Hypr and Vs transfected PS cells at 16 hpt. Cells were fixed with 4% PFA, stained with anti-caspase-3 (red), anti-caspase-8 (green), and DAPI (blue) and imaged on a Zeiss LSM780 confocal microscope. Scale bar: 50 μm. (B) Quantification of caspase-3 and -8 staining determined using Fiji Image J. Bar heights represent the mean ± SEM of three biological replicates. ***, P < 0.0001 from WT Hypr determined using a two-tailed Student's t test with Welch’s correction.