FIG. 2.

Occurrence of the AcrE protein in the mutants DK4201 and DK3402. Envelope preparations containing 40 μg of protein were electrophoresed on a 0.1% SDS–10% (wt/vol) polyacrylamide gel. Arrows indicate the positions of AcrA and AcrE. Proteins were detected by staining with Coomassie brilliant blue R-250 (A) or by immunostaining with antiserum against AcrA (B). Lanes: 1, OST5500; 2, DK4201; 3, DK3402.