TABLE 2.
Solvent resistance of OST5500 and its derivatives
| Strain | Plasmid | Growth on LBGMg agar overlaid witha:
|
||||||
|---|---|---|---|---|---|---|---|---|
| N (5.5) | O (4.9) | CO (4.5) | DE (4.2) | H (3.9) | CH (3.4) | pX (3.1) | ||
| OST5500 | None | + | − | − | − | − | − | − |
| pLAcrAB | + | + | NDb | + | ± | − | − | |
| pLAcrA | + | − | ND | − | − | − | − | |
| pLAcrB | + | + | ND | + | − | − | − | |
| DK4201 | None | + | + | + | + | − | − | − |
| DK3402 | None | + | + | + | + | + | + | − |
a
A drop of the E. coli cell suspension (5 μl) containing approximately 105 cells was spotted on LBGMg agar medium to form a circle with a diameter of 7 to 8 mm. The surface of the agar was overlaid with the organic solvent indicated. Growth was assessed after the plates were incubated at 37°C for 12 h. Values in parentheses are the log POW values of the organic solvents. Abbreviations: N, n-nonane; O, n-octane; CO, cyclooctane; DE, diphenyl ether; H, n-hexane; CH, cyclohexane; pX, p-xylene; +, confluent growth; ±, poor growth (fewer than 10 small colonies in the circle spotted); −, no growth.
b
ND, not determined.