Skip to main content
. 2022 Sep 21;11(1):2160–2175. doi: 10.1080/22221751.2022.2117101

Figure 3.

Figure 3.

Impact of compromised endolysosomes on SC35M infection. Murine lung tissues were either treated with 250 nM bafilomycin A for 1 h or with 10 µg/mL U18666A for 16 h prior to ex vivo infection with the IAV strain SC25M for 24 h. Control, no pre-treatment, infection; Mock, no pre-treatment, no infection (A) Viral titres detected in lung homogenates (PFU/mL). (B) Expression levels of viral M1 gene in non-treated compared to pre-treated SC35M-infected lungs. Data are expressed as fold changes ± SEM compared to control, the cut-off for detected signal was set to Ct ≤ 35. (C) Scatter plot representation of host cell gene expression levels in SC35M-infected lungs compared to mock (pointed lines). Data of individual mouse lungs are expressed as fold changes with means ± SEM superimposed. Statistical significance of the differences was evaluated by Kruskal-Wallis test on ΔΔCt values followed by Dunn's multiple comparisons test. Data were analyzed by Kruskal-Wallis test followed by a Dunn's multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, n.d.    not detectable.