Table 1.

Primers used for generating mutant furin cleavage site SADS-CoV S.

Primer name	Primer sequences
AYVA forward	5′ AGCCAGCTGGCCTATGTGGCCATCCTGGGC 3′
AYVA reverse	5′ GCCCAGGATGGCCACATAGGCCAGCTGGCT 3`
KSAA forward	5′ AAGAGCGCCGCCTTCGTGGAT 3′
KSAA reverse	5′ ATCCACGAAGGCGGCGCTCTT 3′
AVAA forward	5′ GTGGCCGTGGCCGCCATGACCTTT 3′
AVAA reverse	5′ AAAGGTCATGGCGGCCACGGCCAC 3′
AYVR forward	5′ AGCCAGCTGGCCTATGTGCGC 3′
AYVR reverse	5′ GCGCACATAGGCCAGCTGGCT 3′
AVAR forward	5′ GTGGCCGTGGCCAGAATGACC 3′
AVAR reverse	5′ GGTCATTCTGGCCACGGCCAC 3′
SADS S1 forward	5′ CATCATTTTGGCAAAGAATTCGCCACC 3′
SADS S1130 reverse	5′ AAAAAGATCTGCTAGCTCGAGTTATGC 3′