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. 2022 Sep 21;11(1):2147–2159. doi: 10.1080/22221751.2022.2114852

Figure 2.

Figure 2.

Cross-titration of NGPs with serum pools from CL, Chagas disease, and nonendemic controls. cELISA tests were performed with NGP29b, NGP28b, or NGP30b at concentrations ranging from 50 to 3.13 ng/well, using a pool of sera (n = 15) from patients with active CL, caused by L. braziliensis. Pools of sera obtained from patients with chronic Chagas disease (CD) (n = 15), and from nonendemic healthy controls (NEC) (n = 15) were also evaluated. Each point represents the mean of duplicate values of the relative luminescence units (RLU) obtained for each sample and bars indicate SD. Statistical analysis: two-way Anova with main effects only and Dunnett’s multiple comparison test (with individual variances computed for each comparison). The CL and CD curve were compared with the NEC curve, at 1:400 and 1:800 serum pool dilution. *p < 0.05, **p < 0.01,****p < 0.0001; statistically non-significant differences are not shown.