Figure 3.

Normalized IgG response of sera from patients with tegumentary leishmaniasis (TL) to L. major type-2 GIPL-1-derived NGP29b and GIPL-3-derived NGP28b. cELISA immunoassays were performed using NGPs at 5 ng/well and serum samples (1:800 dilution) from all TL samples (n = 80), with different clinical forms (CL; n = 17; ML n = 16; DL, n = 16; and SC, n = 31) plotted separately; Chagas disease (CD, n = 16); and all non-TL, seemingly healthy controls (NEC + EC; n = 33), also plotted separately (EC, n = 15; and NEC, n = 18). Each point represents the mean of triplicate relative luminescence units (RLU) values normalized to NEC serum pools. The cutoff value (cELISA titer = 1.000), calculated as described in Materials and Methods, is indicated by the continuous green line. Data are represented as violin plots (truncated) of individual points, with median (thick black line) and interquartile range (dotted black lines) values indicated. *p < 0.05, **p < 0.01, ****p < 0.0001, Kruskal Wallis followed by Dunn’s multiple comparison tests. Statistically non-significant differences between serum groups are not shown.