Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 24;17:4481–4496. doi: 10.2147/IJN.S378727

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Wang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Cytotoxic properties of CuO NPs. (A) TEM images of BEAS-2B cells before exposure and (B) after exposure to 25 μg/mL CuO NPs for 6 h. (C) Cell viability assessment of Cu ions (CuCl₂) and CuO NPs in BEAS-2B cells. Cells were exposed to serial concentrations (0, 0.4, 0.8, 1.5, 3.1, 6.25, 12.5, 25, 50, 100, and 200 μg/mL) of Cu ions or CuO NPs, and cell viability was assessed by the CCK-8 assay. (D) Live/dead staining of BEAS-2B cells treated with CuO NPs at 200, 100, 50, 25, 12.5 and 0 μg/mL for 24 h. The live and dead cells were stained with calcein AM (green) and PI (red), respectively. (E) Hemolytic effect of CuO NPs. Mouse RBCs were exposed to CuO NPs for 3 h. Inset picture showing hemoglobin appearance (red) in the RBC solution. (F) CLSM images of BEAS-2B cells reflecting intracellular ROS (DCF, green).