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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Aging Cancer. 2022 Apr 19;3(2):116–129. doi: 10.1002/aac2.12049

Figure 4. Second harmonic generation (SHG) microscopic analysis of tumor-naïve omenta from young and aged mice.

Figure 4.

Omenta (n=5 per cohort) were obtained from (A) young (Y) or (B) aged (A) mice and the collagen was imaged with second harmonic generation (SHG) microscopy (25x; inset 100x). Aged omenta have longer, linear fibers (arrows) and thicker banding of fibers (arrowheads) relative to young omenta. Images were analyzed with ImageJ for (C) anisotropy, a measure of linearity (p=0.01); (D) total collagen (p=0.01); and (E) intensity of the SHG signal (p=0.01). Error bars represent standard error of mean and p-values were determined by Student’s t-test.